Recovery Accuracy

Objective:

Characterize Ranger Technology accuracy over multiple size targets

Experiment:

Two genomic DNA samples (Sample A and B) were sheared on a Covaris E210 to produce two different fragment length distribution profiles. These samples were split into aliquots and were run on the Ranger, which recovered fractions ranging from 175 to 1100 bp:

Sample A (100 – 700 bp)

  • Aliquot 1: 175 – 225 bp
  • Aliquot 2: 225 – 275 bp
  • Aliquot 3: 275 – 325 bp
  • Aliquot 4: 325 – 375 bp

sample_A
Sample A Input. All electropherograms produced with Agilent Bioanalyzer 2100.

sample_A_alliquots
Aliquots from Sample A. Peak height does not reflect recovery yields.

Sample B (200 – 1500 bp)

  • Aliquot 1: 400 – 600 bp
  • Aliquot 2: 600 – 800 bp
  • Aliquot 3: 900 – 1100 bp

sample_B
Sample B Input

sample_B_alliquots
Aliquots from Sample B. Peak height does not reflect recovery yields.