Determine accuracy and recovery yields for size selection of large fragments using the Ranger
Input genomic DNA was enzymatically fragmented to produce a distribution of DNA fragments that ranged in size from ~1 kbp to over 17 kbp. Roughly 2 ug of this input DNA was loaded onto the Ranger, which targeted recovery of DNA from 9 kbp to 13 kbp. Pre and post size selection fractions were characterized by a Bioanalyzer 2100 (Agilent Technologies).
Four replicate 20 ul aliquots were created for each of 10 kb and 20 kb NoLimit DNA fragments (Thermo Scientific, # SM1751, # SM1541). Replicates #3 and #4 from each set were run on the Ranger, which targeted the entirety of each band for recovery. The Ranger-processed replicates were concentrated, eluted in 17uL, and all four replicates were quantified using a Qubit Fluorometer (Life Technologies) to characterize recovery of these large fragments.